Scarlets full-back Liam Williams and prop Rhodri Jones are both handed first Test match appearances, together with 19-year-old Cardiff wing Harry Robinson.
Robinson's Blues colleague Martyn Williams, meanwhile, remains on course to win his 100th cap after being included among the replacements.
Williams is set to join Welsh centurions Stephen Jones and Gareth Thomas by reaching the three-figure landmark.
Scarlets hooker Matthew Rees will captain a Wales side that includes a number of fellow Grand Slam and Six Nations winners this season such as locks Alun-Wyn Jones and Ian Evans, plus number eight Ryan Jones.
And there are returns to the Test arena for Ospreys centre Andrew Bishop and fly-half Dan Biggar, who both played key roles in last Sunday's RaboDirect PRO12 title-clinching victory over European champions Leinster in Dublin.
Williams apart, the bench also includes uncapped Scarlets centre Adam Warren for Wales' final game before embarking on a three-Test tour to Australia.
A group of players are set to leave for Australia tomorrow with assistant coach and defence specialist Shaun Edwards. That list includes star names such as Sam Warburton, Mike Phillips, George North, Adam Jones, Dan Lydiate and Toby Faletau.
They will step up preparations for the first Test in Brisbane, with the remaining tour squad members arriving Down Under next Monday.
"We have picked a side on form, with an eye on bringing players through for the future, but very much with the intention of getting the summer campaign off to a winning start," Howley said.
"We have an opportunity against the Barbarians to get an important summer series off to a winning start.
"We have momentum from the Six Nations, and the Ospreys have added to that with a great win in our domestic competition.
"We are now looking for the next step and a victory on home soil against a formidable Barbarians team before we set off for Australia."
The final Australia tour squad will total 34 players, with the last few names confirmed after Saturday's Millennium Stadium encounter.
In addition to facing the Wallabies in Brisbane, Melbourne and Sydney between June 9 and 23, Wales also face a midweek appointment with the Canberra-based Brumbies.
Wales team to play the Barbarians at the Millennium Stadium on Saturday (kick-off 1400 BST):
Liam Williams (Scarlets); H Robinson (Cardiff Blues), A Bishop (Ospreys), J Hook (Perpignan), A Brew (Newport Gwent Dragons); D Biggar (Ospreys), Lloyd Williams (Cardiff Blues); R Gill (Saracens), M Rees (Scarlets, capt), Rhodri Jones (Scarlets), A-W Jones (Ospreys), I Evans (Ospreys), J Turnbull (Scarlets), J Tipuric (Ospreys), Ryan Jones (Ospreys).
Replacements: R Hibbard (Ospreys), P James (Ospreys), A Shingler (Scarlets), M Williams (Cardiff Blues), R Webb (Ospreys), A Warren (Scarlets), W Harries (Newport Gwent Dragons).
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