Hamilton and Sharples have been released from international duty from Scotland and England respectively - and they go straight in to the starting XV.
Director of rugby Nigel Davies has opted to keep the majority of the side from the win over London Welsh last time out, with Dan Murphy and Sione Kalamafoni also handed starts.
Ben Morgan is the notable absentee, though, after he was handed the England No 8 jersey to face South Africa.
Sale will welcome back Rob Miller to their line-up for the trip to Kingsholm.
The talented full-back replaces the benched Cameron Shepherd, while Will Addison and Sam Tuitupou will team up in the midfield.
Andy Powell, Ross Harrison and Tommy Taylor are added to the pack, with Joe Ward, Eifion Lewis-Roberts and James Gaskell named among the replacements.
Gloucester: 15 Rob Cook, 14 Charlie Sharples, 13 Mike Tindall, 12 Billy Twelvetrees, 11 Shane Monahan, 10 Freddie Burns, 9 Jimmy Cowan, 1 Dan Murphy, 2 Huia Edmonds, 3 Rupert Harden, 4 Will James, 5 Jim Hamilton (capt), 6 Tom Savage, 7 Akapusi Qera, 8 Sione Kalamafoni. Replacements: 16 Koree Britton, 17 Nick Wood, 18 Shaun Knight, 19 Peter Buxton, 20 Matt Cox, 21 Dan Robson, 22 Tim Molenaar, 23 Martyn Thomas.
Sale Sharks: 15 Rob Miller, 14 Charlie Amesbury, 13 Will Addison, 12 Sam Tuitupou, 11 Mark Cueto, 10 Danny Cipriani, 9 Dwayne Peel, 1 Ross Harrison, 2 Tommy Taylor, 3 Tony Buckley, 4 Fraser McKenzie, 5 Kearnan Myall, 6 Richie Vernon, 7 David Seymour (capt), 8 Andy Powell. Replacements: 16 Joe Ward, 17 Eifion Lewis-Roberts, 18 Vadim Cobilas, 19 Tom Holmes, 20 James Gaskell, 21 Will Cliff, 22 Nick Macleod, 23 Cameron Shepherd.
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The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
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