The Ospreys are currently fifth in the RaboDirect PRO12 table while Gloucester are fourth in the Aviva Premiership after losing away to Harlequins last time out.
Marc Thomas and Will Price will make their first Ospreys starts.
Scott Otten, Nicky Smith, Nicky Thomas, Rhodri Hughes, Arthur Ellis, Sam Davies and Nathan Edwards will all make their Ospreys debuts if they appear off the bench.
Gloucester will field an inexperienced team. In the backs, the likes of Martyn Thomas, Shane Monahan, Tim Molenaar, Tim Taylor and Dan Robson have first-team experience while there is a debut for centre Drew Locke.
In the pack the experience of Tommaso d'Apice, Dario Chistolini, Matt Cox and skipper Peter Buxton is backed up by a number of promising young forwards, including current England U20 squad members Elliott Stooke and Ross Moriarty.
Ospreys: Ross Jones, Tom Grabham, Ben John, Jonathan Spratt, Will Price, Matthew Morgan, Rhys Webb, Marc Thomas, Matthew Dwyer, Joe Rees, Lloyd Peers, James King, George Stowers, Sam Lewis (cpt), Morgan Allen.
Replacements: Scott Otten, Nicky Smith, Nicky Thomas, Rhodri Hughes, Arthur Ellis, Tom Habberfield, Sam Davies, Nathan Edwards
Gloucester: Martyn Thomas; Shane Monahan, Drew Locke, Tim Molenaar, Ian Clark; Tim Taylor, Dan Robson; Yann Thomas, Tommaso d'Apice, Dario Chistolini; Elliott Stooke, Will Graulich; Ross Moriarty, Matt Cox, Peter Buxton (cpt).
Replacements: Koree Britton, Dan Murphy, Tom Heard, Ed King, Ben Sparks, Dave Lewis, Billy Burns, Steph Reynolds
Gilbert has released a new line of rugby cleats. The Gilbert Virtuo 8S is part of the exciting new product. Check it out.
The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
The Nike Tiempo is a solid rugby cleat and one of few styles still made from full-grain natural leather.
The Gilbert Blitz 8S rugby cleat is a great cleat at a great price of $69.99. Get a new pair of cleats today.
A cool looking all black rugby cleat with the high performance adidas is known for. Get in the Gear!
Wear the crest of the British and Irish Lions on your t-shirt. A great look for the summer.
The All Blacks Performance t-shirt is black with hints of blue from the training jersey. Very Cool.
The New Zealand All Blacks training jersey for 2013/14. Get in the Gear!
The USA Rugby Pro Alternate rugby jersey is perfect for any fan of the Eagles. Get yours to wear during the summer Test matches.
The NEW All Blacks 2013/14 jersey has arrived at World Rugby Shop. Dare to wear the colors of the All Blacks.