The Cherry and Whites, who are defending their title, were edged 13-11 by the Sharks in the Premiership last weekend, and Tom Savage, Will James, Brett Deacon, Luke Narraway, Henry Trinder, Charlie Sharples and Jonny May are the men who retain their places.
Dan Murphy, Matias Cortese and Dario Chistolini make up a new-look front row, while the experienced James Simpson-Daniel and Lesley Vainikolo also start.
Sale have also opted to change half their side and hand centre Luther Burrell a competitive debut.
Scotland internationals Richie Vernon and Alasdair Dickinson are also handed first starts, while Tony Buckley and Johnny Leota wait on the bench to take their bows.
Sharks' Executive Director of Sport Steve Diamond said: "We have selected our strongest possible side but it will be a different challenge from last weekend, as there will be 13,000 people, including the Shed, screaming for Gloucester."
Gloucester: May, Sharples, Trinder, Vainikolo, Simpson-Daniel, Burns, Lewis; Murphy, Cortese, Chistolini, Savage, James, Deacon, Cox, Narraway (capt).
Replacements: S Lawson, Y Thomas, Knight, Hamilton, Strokosch, R Lawson, Taylor, Voyce.
Sale: Miller, Brady, Higgins, Burrell, Amesbury, Macleod, Mathie; Dickinson, Ward, Cobilas, McKenzie, Gaskell, Vernon, Seymour (capt.), Easter.
Replacements: T Taylor, Imiolek, Buckley, Holmes, Myall, Cliff, Leota, Addison.
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The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
The Nike Tiempo is a solid rugby cleat and one of few styles still made from full-grain natural leather.
The Gilbert Blitz 8S rugby cleat is a great cleat at a great price of $69.99. Get a new pair of cleats today.
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The NEW All Blacks 2013/14 jersey has arrived at World Rugby Shop. Dare to wear the colors of the All Blacks.