Roberts originally picked up a knee problem in the Heineken Cup win over Racing Metro in November and suffered another injury to the same knee in Friday's 31-3 defeat against Glasgow and needs surgery on his anterior cruciate ligament.
He will miss this weekend's daunting Heineken Cup quarter-final against Leinster, with Dafydd Hewitt or Gavin Evans set to deputise.
Gavin Henson would have been a potential replacement but he was sacked by the Blues on Monday for his alcohol-fuelled behaviour on the flight back from Glasgow.
A Blues statement read: "Cardiff Blues can confirm that Blues and Wales centre Jamie Roberts will require surgery on his knee and will miss the rest of the season."
Gilbert has released a new line of rugby cleats. The Gilbert Virtuo 8S is part of the exciting new product. Check it out.
The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
The Nike Tiempo is a solid rugby cleat and one of few styles still made from full-grain natural leather.
The Gilbert Blitz 8S rugby cleat is a great cleat at a great price of $69.99. Get a new pair of cleats today.
A cool looking all black rugby cleat with the high performance adidas is known for. Get in the Gear!
Wear the crest of the British and Irish Lions on your t-shirt. A great look for the summer.
The All Blacks Performance t-shirt is black with hints of blue from the training jersey. Very Cool.
The New Zealand All Blacks training jersey for 2013/14. Get in the Gear!
The USA Rugby Pro Alternate rugby jersey is perfect for any fan of the Eagles. Get yours to wear during the summer Test matches.
The NEW All Blacks 2013/14 jersey has arrived at World Rugby Shop. Dare to wear the colors of the All Blacks.