O'Connor, who came off the bench and scored a try and three conversions in the 32-6 win over Italy, replaces the injured Digby Ioane on the wing.
O'Connor, who served a one-match ban at the end of Tri-Nations for breaching team protocol, will also take over the kicking duties from Quade Cooper.
Coach Robbie Deans also welcomes back winger Drew Mitchell to the squad - with the Waratahs ace having been sidelined since suffering a serious ankle injury back in April.
Mitchell, who scored five tries during the 2007 Rugby World Cup, comes in on the bench.
"Drew has worked hard within the group, he has the background with us and is ready for this opportunity," said Deans.
"While it is disappointing for Digby that he is going to be on the sidelines for a while, we are confident he will be available to us again before too long. His timelines will depend on how his rehabilitation goes, but the operation on Tuesday night went well."
Australia: 15 Kurtley Beale, 14 James O'Connor, 13 Anthony Fainga'a, 12 Pat McCabe, 11 Adam Ashley-Cooper, 10 Quade Cooper, 9 Will Genia, 8 Radike Samo, 7 David Pocock, 6 Rocky Elsom, 5 James Horwill (capt), 4 Dan Vickerman, 3 Ben Alexander, 2 Stephen Moore, 1 Sekope Kepu.
Replacements:16 Tatafu Polota Nau, 17 James Slipper, 18 Rob Simmons, 19 Ben McCalman, 20 Scott Higginbotham, 21 Luke Burgess, 22 Drew Mitchell.
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The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
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